Phototoxicity Evaluation by Human 3D Dermal Model

Creative Biolabs has established a leading comprehensive platform for a series of 3D dermal models for dermal phototoxicity testing, formulation safety, and efficacy testing, providing high-quality phototoxicity evaluation services.

Phototoxicity Overview

Definition of Phototoxicity	Localized or systemic non-immunotoxic effects occur when the skin is exposed to a specific light source (e.g., sunlight or certain artificial light sources) after exposure to a photosensitizing or phototoxic substance.
Common photosensitizing/phototoxic agents	Medications, such as antibiotics, antidepressants, diuretics, and photosensitization enhancers Cosmetics and personal care products, e.g., perfumes, hair dyes, sunscreens, and preservatives added to skin care products Plant extracts and their derivatives, e.g., citrus fruit juices.
Skin phototoxicity symptoms	Erythema, itching, irritation, inflammation and edema.
Factors affecting the degree of phototoxic reaction	Degree of sensitivity to phototoxicity Light intensity and light dose Properties of the phototoxic materials
Advantages of phototoxicity assay by 3D dermal model	The model features structural, cytokine secretion and metabolic capabilities comparable to those of human skin tissues. Toxicity predictions based on the 3D model exhibit a higher correlation with in vivo studies and a lower false-positive rate than 2D culture conditions. As an alternative to conducting skin phototoxicity assays using animal models.

Phototoxicity Evaluation Protocol by Human 3D Dermal Mode

Evaluation principle

Comparison of skin cytotoxicity resulting from the application of a given amount of the tested materials with and without exposure to non-toxic UVA visible light.

Evaluation model

3D dermal model

Tested materials preparation

Liquid subjects: can be directly used as a raw liquid or diluted with oil or H₂O.
Solid subjects: after grinding into powder, fully moistened with oil or H₂O.
Cosmetic subjects: generally applied in raw cream or liquid.

Tested materials preparation

Liquid subjects: can be directly used as a raw liquid or diluted with oil or H₂O.
Solid subjects: after grinding into powder, fully moistened with oil or H₂O.
Cosmetic subjects: generally applied in raw cream or liquid.

Testing conditions

The concentration of the tested materials applied should not induce a skin irritation reaction (can be determined through pre-testing).
UV light: 320 nm-400 nm UVA
Duplicates: N=2 tissues/ condition

Testing controls

Background control for the subjects: oil/H₂O.
Positive control: chlorpromazine, promethazine hydrochloride, 8-methoxy psoralen, and other known phototoxic compounds.

Testing indicators

Relative tissue viability
Inflammatory factor release.

Evaluation Workflow

Evaluation Workflow (Creative Biolabs)

Fig. 1 Dermal phototoxicity testing of dicloran and intermediate photoproducts.¹

Creative Biolabs values the close proximity, stability, and reusability of the human 3D dermal model and provides phototoxicity testing services utilizing it to meet customer needs for phototoxicity testing of diverse materials. Please contact us to obtain a comprehensive service.

Reference

Xu, W.; et al. Potential risk to human skin cells from exposure to dicloran photodegradation products in water. Environ Int. 2018, 121(Pt 1): 861-870.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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Research Model

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