3D Biology Based Plasmacytoid Dendritic Cells Activation Assay

Dendritic cells are the most potent antigen-presenting cells in the body. Based on their origin and phenotype, dendritic cells are primarily divided into myeloid dendritic cells and plasmacytoid dendritic cells (pDCs). These two types of dendritic cells differ in their secretion of cytokines, types of cytokines, and immunoregulatory functions. Due to the unique functions of pDCs in antiviral immunity and immune regulation, the activation assay of pDCs is crucial in the development of drugs and vaccines. Creative Biolabs offers 3D biology-based pDCs activation assay services, aiming to assist clients in accurately evaluating the immune activity and efficacy of candidates.

Functions of Plasmacytoid Dendritic Cells (pDCs)

pDCs exhibit plasmacytoid morphology and possess unique immunoregulatory functions.

Immune Response

pDCs are activated by recognizing antigens through Toll-like receptor 9 expressed on their surface. Activated pDCs modulate the function of other immune cells, thereby mediating immune responses or promoting immune tolerance.

Antiviral Immunity

pDCs play a critical role in antiviral immunity. They can recognize viral RNA, DNA, or immune complexes containing RNA/DNA and secrete various types of interferons to inhibit viral replication and spread.

Regulation of Tumor Immunity

pDCs have a dual role in tumor immunity. On one hand, they can recognize tumor-associated antigens and activate themselves and other immune cells to counteract tumor cells. On the other hand, prolonged exposure to interferon-I can induce immune suppression by upregulating the expression of Interleukin 10 and indoleamine 2,3-dioxygenase in dendritic cells.

Immunological Memory

pDCs participate in regulating the memory response of adaptive immunity, playing a crucial role in the establishment of long-term immune protection.

Fig.1 The mechanism of pDC activation.1

3D Biology-Based pDCs Activation Assay

3D models, including the 3D spheroid model, organ-on-a-chip model, 3D ex vivo human tissue model, and precision-cut tissue slice model, which are known for their ability to closely mimic the in vivo environment and cell-cell interactions, have garnered significant attention and have been widely applied in mechanistic studies and drug development for various diseases. To meet the preclinical research needs of our clients, Creative Biolabs offers a more realistic and reliable 3D biology-based assay for activating plasmacytoid dendritic cells, catering to diverse research demands.

Immunological Research

The pDCs activation assay enables the evaluation of pDCs' responsiveness and immunoregulatory capacity towards stimuli, facilitating the investigation of immune response mechanisms, disease pathogenesis, and the efficacy of immunotherapies.

Drug Development

The pDCs activation assay plays a vital role in immunotherapy and vaccine development. It can be utilized to assess the activation effects of candidate drugs or vaccines on pDCs, aiding in the screening and optimization of drug or vaccine designs.

Immunogenicity Evaluation

Certain drugs or compounds may elicit adverse effects on the immune system. The pDCs activation assay can be employed to evaluate the immunogenic response of pDCs to drugs, thus assisting in predicting potential adverse effects of drugs on the immune system.

3D Biology-Based pDCs Activation Assay Service Workflow

1. Experimental Design
2. pDCs Acquisition
3. Construction of 3D Biology-Based pDCs Model
4. Drug Administration: Cell phenotype markers, cytokine release, gene expression, and other relevant indicators
5. Evaluation of pDCs Activation
6. Data Analysis and Interpretation
7. Results Interpretation and Discussion

With a highly skilled team and extensive experience, Creative Biolabs assists clients in gaining an in-depth understanding of disease mechanisms, therapeutic effects of drugs or vaccines, and immunogenicity evaluations based on the delivered results. We provide excellent support for your research and development endeavors.

If you require more valuable data on pDCs activation levels, please contact us for more detailed information. We are dedicated to constructing 3D biology-based pDC models and offering comprehensive activation assessment services.

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services