3D Biology Based Proteomic Analysis Service

Proteins play a vital role in living organisms and have a variety of activities, and proteomics is the comprehensive study of proteins. Creative Biolabs, in addition to the multi-omics analysis based on DNA and RNA, can qualitatively and quantitatively profile proteins or post-translational modification sites in 3D biology-based samples, which are the more popular experimental models in animal development or disease occurrence.

What Is Proteomics?

The proteome of an organism means the whole collection of proteins made or altered. Proteomics is the high-throughput study of the protein makeup of cells, tissues, or organisms, and is a crucial part of functional genomics. It involves the research of proteomes from the general level of protein composition, structure, and activity. Proteomics frequently refers particularly to mass spectrometry and protein purification, and the former is the most potent technique for analyzing proteomes.

What Is 3D Biology?

3D biology models are attractive for scientific researchers because of the similarity between models and real tissues. The 3D aggregates can highly recapitulate the cell composition, structure, and microenvironment and recur the development process of the real tissue/organ growth or disease occurrence.

3D Biology Based Proteomics

The proteomics analysis on 3D biology-based samples can

• Detect protein location, quantity, modification, or binding specificity of samples with different experimental conditions or treatments.
• Identify the site and pace of protein synthesis and deterioration at a given moment in addition to steady-state abundances.
• Profile the proteomes, quantify protein biomarkers and post-translational modifications (PTM), and identify the direct targets or indirect effects when conditions change like drug treatment.
• Help with exosome research like profiling and identifying signatures and biomarkers of the exosome.

The results based on 3D biology are more convincible and can lead to huge improvements in disease diagnosis and therapy. For example, 3D biology-based proteomics can identify and quantify PTMs associated with drug response and detect the drug effects at the protein level.

How about The Procedure?

• In the beginning, the total protein will be extracted from your aggregates or organoids. An antibody-based strategy like immunoprecipitation or a size-based method like SDS PAGE can help to narrow down the investigation range.
• Then, the proteins will be digested into peptides, which are separated by high-performance liquid chromatography (HPLC).
• Next, the separately enriched peptides will be analyzed by mass spectrometry and quantified based on associated software.
• Finally, the data will be depicted according to different research goals such as the gene ontology database for functional enrichment analysis.
• Additional steps such as quality control of raw data, sample normalization, and correction of batch-related effects will be involved during the procedure to generate more promising and convincing results.

Fig.1 The workflow of proteomic analysis. (Creative Biolabs)

Creative Biolabs provides top-notch services for your 3D biology based proteomic analysis. Our committed analyst supported by an experienced team will organize all the data, choose the best statistical strategy, and offer a biological interpretation of the consequence. For more details, please feel free to contact us about your project.

3D Models Provided by Creative Biolabs

• 3D Spheroid Model
• Organ-on-a-chip Model
• 3D Ex Vivo Human Tissue Model
• 3D Precision-Cut Tissue Slice Model
• 3D Organoid Model

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services