3D Biology Based RNA Sequencing Service

The coding, decoding, regulation, and expression of genes are all biological processes that depend on the polymeric molecule known as RNA. An essential technique for the transcriptome-wide investigation of many facets of biology is RNA sequencing (RNA-Seq). Being fully aware that 3D biology has a promising future in science, Creative Biolabs integrates the RNA-Seq method with our 3D biology model to provide our clients all over the world with a comprehensive and tailored RNA-Seq solution.

What Is RNA-Seq?

RNA-Seq is a sequencing method that examines the constantly changing cellular transcriptome to determine the presence and amount of RNA in a biological sample at a certain time. With the benefits of next-generation sequencing, RNA-Seq makes it possible to examine alternatively spliced genes, post-transcriptional alterations, gene fusions, mutations, single-nucleotide polymorphism, and changes in gene expression over time. It also can detect the variations in gene expression in response to different treatments or groups, which gives researchers insight into previously unnoticed alterations taking place in disease states, in response to medicines, in various environmental settings, and across a wide range of other study types without being constrained by prior knowledge.

3D Biology Based RNA-Seq

Applying RNA-Seq to different kinds of 3D biology models, we will have comprehensive and detailed knowledge about our aggregates. And, we can be told how these aggregates represent the intrinsic and extrinsic characteristics with a comparison of their corresponding structure in vivo, which is perfect guidance to refine our 3D biology models research such as discovering promising biomarkers, increasing the clinical relevance of in vitro trials, and improving the investigation for human diseases or development because of the rare research materials.

3D biology-based RNA-Seq has many advantages, such as:

How about The Procedure?

This procedure extracts total RNA from your 3D biology-based samples. Different methods will be taken into consideration for the enrichment of the RNA we are interested in. For example:

• Hybridization with poly(dT) oligomers helps to collect the coding RNA, desired transcripts will be enriched when the specific probes are used.
• Ribosomal RNA is depleted before the sequencing of lncRNA and circRNA, furthermore, the latter needs an additional RNase R treatment to remove the linear transcripts.
• MicroRNAs can be enriched by their small size.

After RNA enrichment, we generate the cDNA library by reverse transcription and library construction. Finally, sequencing and bioinformatics analysis help us to further understand the important information.

Fig.1 The workflow of RNA sequencing. (Creative Biolabs)

For your 3D biology-based RNA sequencing needs, Creative Biolabs offers top-notch and qualified services. Each stage is carried out in accordance with a rigorous scientific standard and precise design to ensure excellent study outcomes. Please feel free to get in touch with us for additional information about your 3D biology project.

3D Models Provided by Creative Biolabs

• 3D Spheroid Model
• Organ-on-a-chip Model
• 3D Ex Vivo Human Tissue Model
• 3D Precision-Cut Tissue Slice Model
• 3D Organoid Model

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services