3D Biology Based Single Cell Sequence Service

Single-cell sequencing plus the improvements in technique and algorithm provide an attractive and promising tool for revealing the full complexity of cellular diversity and approaching a series of seemingly inaccessible problems. Creative Biolabs, with rich experience in single-cell sequencing, has established reliable methods to solve your complex problems in popular 3D biology research areas. We provide a comprehensive and specialized service for our clients worldwide.

What Is Single-Cell Sequencing?

With well-refined next-generation sequencing methods, single-cell sequencing can analyze the sequence data from individual cells and give a better knowledge of the function of a single cell in relation to its microenvironment and a deeper resolution of cellular distinctions. It can also be used to investigate the cell-lineage associations, functions of individual cells, and samples of heterogeneous natures compared with bulk sequencing.

Fig.1 Single-cell sequencing can illustrate the heterogeneity whereas bulk sequencing can’t. (Schatten, 2021)

Function of Single-Cell Sequencing

Usually, single-cell sequencing can be classified into single-cell RNA genome sequencing (scRNAseq) and single-cell DNA sequencing (scDNAseq) by differently involved molecules. The scRNAseq technique detects the data of gene expression at the single cell level, which has become the gold standard for identifying cell states and cell phenotypes and may reveal previously unknown uncommon cell types within a group of cells. Mammalian systems have used scDNAseq extensively to explore both healthy physiology and illness. For instance, sequencing the DNA of a few cancer cells might uncover their mutation information and tumor genomic heterogeneity.

3D Biology Based Single-Cell Sequencing

3D biology models show great promise for producing realistic tissue and disease models. Compared to 2D cell culture, the aggregates increase the cell-cell interactions and mock the oxygen and nutrient gradient during cell culture. When exposed to the drug, it can recapitulate the in vivo microenvironment and create the drug gradient from outside to inside. The application of single-cell sequencing on 3D cell models can faithfully recapitulate the gene expression and illustrate the gene regulatory network features in vivo, which provides a direct and in-depth understanding of the cells we are interested in, raises the clinical therapeutic relevance of trials, helps us to discover clinically meaningful biomarkers, and speeds up the research of developmental biology and human diseases.

Single-Cell Sequencing Workflow

Usually, three steps are involved during single-cell sequencing.

• The samples usually are single-cell suspensions derived from your aggregates with a defined cell density. Reverse transcription is involved in scRNAseq.
• Raw data will be generated after library construction and sequencing. During the procedure, three different kinds of quality control are involved to guarantee high-quality research outcomes.
• Finally, bioinformatics analysis helps us better understand the sequencing results.

Fig.2 The procedure of single-cell sequencing. (Creative Biolabs)

Creative Biolabs provides high-quality and professional services for your 3D biology based single-cell sequencing. Each step is performed in agreement with a high scientific standard and meticulous design to ensure high-quality research results. For more details, please be free to contact us about your project.

3D Models Provided by Creative Biolabs

• 3D Spheroid Model
• Organ-on-a-chip Model
• 3D Ex Vivo Human Tissue Model
• 3D Precision-Cut Tissue Slice Model
• 3D Organoid Model

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services