3D Biology Based T Cell Proliferation Assay

Creative Biolabs' 3D ex vivo model-based analysis platform simulates and replicates the state of the immune system in vivo, supporting a wide range of assays including T-cell proliferation.

General Process

1. Design and construction of 3D biological models. Based on models that are composed of multiple micro-tissue modules, micro-coupled models that mimic the human immune system, including T cells, antigen-presenting cells, and other relevant cells, can be produced using microfluidic techniques and biomaterials in T cell proliferation assays.
2. Cell culture and manipulation. T cells and other cells are colonized into the model and appropriate culture conditions, such as nutrients, oxygen, and temperature, are provided to maintain model stability.
3. Stimuli injected into the model. Cells in the model can be exposed to different stimuli, such as antigens, pathogens, drugs, etc., to simulate a specific immune state and to observe the proliferative response of T cells.
4. Monitoring and analysis of cell proliferation. The proliferation can be monitored and analyzed in real-time and quantitatively by imaging analysis, fluorescent labeling, and quantitative PCR. These analysis methods can be used to understand the proliferation rate, differentiation status, and functional changes of T cells.

Fig. 1 Examples of proliferation detection by flow cytometry.¹

Features

• Realistic simulation. Models can provide a more realistic physiological environment and can more realistically simulate human cellular interactions and the function of the immune system.
• Fast and high throughput. Compared with traditional cell culture methods and animal models, T cell proliferation assays based on 3D in vitro models can perform multiple experiments in a short time and with high efficiency.
• Precise control of conditions. The culture conditions, stimulant type and concentration, and cell interactions can be flexibly controlled to meet specific experimental needs.
• Reduce the need to use animals. Models can replace some of the experiments performed using animals, reducing the number of animal experiments and being more ethical.

Applications

• Immunology research. To study the function and activity of immune cells, assess the effects of different stimuli on T cells, and investigate the mechanisms and processes of the immune response.
• Tumor immunotherapy monitoring. The effect of immune intervention strategies on tumors can be assessed and the intensity of the immune response can be judged.
• Evaluate potential diagnostic strategies. It can help to develop diagnostic strategies for immune system-related diseases and determine the functional status of the immune system.

Creative Biolabs specializes in providing a wide range of 3D biology-based assays including T cell proliferation to meet customized project purposes. Please contact us to discuss your project.

Related Sections:

• 3D Biology Based Multi-Omics Analysis Services
• 3D Biology Based Immune Response Analysis Services
• 3D Biology Based Target Analysis and Validation Services