3D Biology Based T Cell Suppression Assay

The 3D biology-based approach has the advantage of being highly simulated and can be used to detect the effects of different factors on T cell suppression, including antitumor drugs, immunosuppressants, cytokines, etc. Creative Biolabs can provide 3D biology-based T cell suppression assay services, regardless of your interest in the research it involves.

3D Biology Based T Cell Suppression Assay Protocol

Select appropriate cell types and vectors to construct a 3D in vitro biological model of T cells and other relevant cells on vectors.
Add molecules that need to be tested, such as drugs, T-cell inhibitors, etc.
Detection of T cell suppression or death by expression of specific markers or fluorescent probes, etc.
Assess the degree of inhibition and differential sensitivity to the tested molecules.
Count and compare the differences in responsiveness to suppression when different samples and experimental conditions are applied to key regulatory cells.

Detection of T cell suppression. Fig. 1 Basic Tregs suppression assay setup.¹

Advantages of 3D Biology Based T Cell Suppression Assay

3D biological models can simulate the natural environment of T cells as a whole compared with the traditional model, which makes the experiment more realistic.
Different processes of T cell suppression and death can be simulated under different conditions to obtain more detailed data.
It can be used for large-scale screening of novel antitumor drugs or immunosuppressants to improve drug screening efficiency.
It can be used as an alternative method to replace traditional animal experiments and reduce the dependence on animals.

Applications

To assess the effect of novel antitumor drugs or immunosuppressive agents.
To detect the role of different cytokines or extracellular matrix in the regulation of the T cell suppression mechanism.
To assess the immune response between graft and host and to investigate the role of T cell suppression in graft rejection.
To explore the role of T cell suppression mechanisms in infectious and immune-related diseases.

3D model-based T cell suppression assays can simulate the overall cellular microenvironment and assess the role of different factors on T cells with more accurate responses and higher screening efficiency for immunomodulatory strategies. Following your experimental queries, Creative Biolabs can create the best T cell assays, or a variety of alternatives and add-ons, taking into account technical considerations as necessary. Please contact us to describe your requirements.

Reference

Irekeola, Ahmad Adebayo, et al. "Technical Considerations in Ex Vivo Human Regulatory T Cell Migration and Suppression Assays." Cells 9.2 (2020): 487.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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Research Model

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