Pigmentation & Melanogenesis Evaluation by Reconstructed Human Epidermis (RhE) Model

Pigmentation & Melanogenesis represents the protective response of the skin in the face of ultraviolet radiation and is also one of the important targets for testing the skin irritation potential of compounds. Creative Biolabs has launched a reconstructed human epidermis (RhE) model for the assessment of pigmentation and melanogenesis and related services to support the prediction of compound or cosmetic skin reactions.

Pigmentation & Melanogenesis

The enzymatic actions of tyrosinase, TYRP1, and DCT facilitate the conversion of tyrosine into melanin within melanocytes. Melanin serves the dual purpose of ensuring a uniform skin complexion and offering protection against DNA damage caused by ultraviolet radiation. External stimuli may potentially disrupt melanin production and deposition patterns.

By employing meticulous biometric assessment protocols and conducting investigations into intricate interactions, we are capable of meticulously documenting even the slightest variations in melanin levels amidst a vast array of hundreds of constituents, thereby deciphering potential risks.

Creative Biolabs’ RhE Pigmentation & Melanogenesis Evaluation

Through the combination of patient-derived epidermal keratinocytes and melanocytes obtained via meticulous skin biopsies, we have engineered a pigment-reconstructed tissue model. This avant-garde model affords us the ability to comprehensively appraise the impact of diverse compounds or stimuli on the modulation of human skin responses, pigment deposition, and melanin biosynthesis within an in vitro setting.

Fig 1. Histological detection of melanogenesis.1

Key Features

Significantly, the assessment of skin irritation potential elicited by test compounds is conducted via the employment of the MTT tissue viability assay, thus furnishing a robust foundation of data substantiating our investigation into the regulatory mechanisms governing pigment deposition. The advantages of our RhE Model include:

Cost-effectively:

Our RhE model provides alternatives to animal testing, screening any potential candidates in a cost-effective way.

Physiological relevance:

Our model exhibits morphological and structural features similar to human skin tissue and allows for the spontaneous generation of melanin particles to simulate deposition processes.

High reproducibility:

The high-throughput testing model ensures consistency and replicability of results, providing accurate predictions and reliable data interpretations.

Customizability:

Through collaborations with various donor organizations, we provide testing samples from different ethnicities, skin colors, or sun-reactive skin types.

What We Provide?

Creative Biolabs is dedicated to providing sophisticated multi-layered RhE models that cater to the diverse testing needs of researchers worldwide, spanning the realms of medical, compound, and cosmetic domains. Our services encompass a spectrum of vital endpoints; for instance, meticulous biotoxicity evaluations conducted via MTT control testing, as well as conclusive screening determinations predicated on exhaustive melanin concentration analyses.

Moreover, our comprehensive suite of physiological skin response research offerings integrates cutting-edge methodologies, thereby ensuring a one-stop solution to satiate your exigent requirements for unraveling the intricacies of cutaneous reactivity.

Our adept team stands poised to deliver bespoke research endeavors meticulously tailored to align with the precise contours of your unique research objectives. Contact us today to discuss your requirements and embark on a successful research partnership.

Reference

Zhang, Y.; et al. Establishment and validation of evaluation models for post-inflammatory pigmentation abnormalities. Frontiers in Immunology. 2022, 13: 991594.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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Research Model

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