Knock-In Organoid Model for Target Profiling Analysis

Gene knock-in is a widely used technique in biomedical research, involving the integration or replacement of exogenous functional genes into specific genomic loci, allowing for stable expression within cells. This powerful method has extensive applications in elucidating gene function, disease modeling, and drug screening. While various knock-in technologies have been proficiently applied in cell and animal models, these knock-in models have limitations in accurately reflecting the physiological phenomena of tissues and organs in vivo. The advancement of human biology research necessitates the establishment of new model systems to provide robust tools for investigation.

Excitingly, organoids have emerged as a significant development in biomedical research. Creative Biolabs combines gene knock-in technology with organoid construction to offer knock-in organoid model construction and research services for target analysis, catering to the needs of customers in target research.

Advantages of Knock-in Organoid Model

Applications of Knock-in Organoid Model

Functional research platform

Knock-in organoid models can be used to evaluate the function and interactions of target genes, uncovering the mechanisms underlying related diseases and potential therapeutic targets.

Efficient drug screening

Knock-in organoid models can be employed for target validation and screening of drugs, assessing the impact of drugs on targets at the tissue level.

Disease modeling

Through knock-in organoid models, gene mutation models related to specific diseases can be constructed, simulating the occurrence and progression of specific diseases.

Precision medicine research

Knock-in organoid models can be customized based on individual genomic information, establishing personalized disease models, and supporting the development of individualized treatments and precision medicine.

Literature Sharing Based on the Knock-in Organoid Model

• Title: Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing
• Research Background: Robust knock-in methods that enable precise integration of exogenous DNA sequences into human organoids are lacking. Although non-homologous end-joining repair mechanisms can force cells to insert new DNA fragments at specific locations, this method is considered prone to errors and therefore not commonly used for gene knock-ins.
• Model Used: Human liver organoids with knock-in of different fluorescent-tagged genes
• Research Findings: The authors successfully labeled microtubules and E-cadherin, key components of the cell division machinery in liver organoids, using knock-in of fluorescent-tagged genes. They studied the process of cell division by tracking these specific components and conducting subsequent functional validation experiments. To their surprise, through these experiments, the authors discovered that TP53 gene mutation plays a crucial role in abnormal cell division in liver cells.

Fig.1 Generation of human hepatocyte reporter organoid lines using CRISPR–homology-independent organoid transgenesis.¹

One-Stop Service Process Based on Knock-in Organoid Model at Creative Biolabs

• Pre-sales consultation
• Experimental design
• Gene knock-in
• Organoid construction
• Knock-in validation
• Extensive functional evaluation
• Data analysis and reporting

Creative Biolabs provides excellent services in the construction and research of knock-in organoid models for target analysis. Our team of experts and cutting-edge technologies make us an ideal partner in this field. If you are seeking high-quality and customized research tools, we highly recommend contacting us for the best service and support. In addition, of course, we also offer the over-expression organoid model, knock-down organoid model, and knock-out organoid.

Related Sections:

• Gene Editing Organoid Based Target Analysis and Validation Services
• Off-Target Screening Cell Microarray Assay