Knock-Out Organoid for Target Profiling Analysis

Gene knock-out is an experimental technique used to eliminate or inactivate a specific gene in cells or organisms. It purposefully alters the DNA sequence or expression of the target gene through various methods such as gene editing technologies, RNA interference, or induced mutations, resulting in the loss of function or inhibition of the target gene. By knocking out specific genes, researchers can uncover the target gene's function and mechanisms of action and determine its impact on cell or organism development, physiological processes, or disease occurrence.

With the emergence of organoids, the combination of gene knock-out techniques and organoid culture has become an important tool in biomedical research and drug development. Creative Biolabs provides gene knock-out organoid model construction and functional research services for target analysis to meet the research needs of clients.

Introduction to the Usage of Knock-out Organoid Models

Target Research Model

By knocking out specific genes, phenotypic changes in organoid models can be observed, inferring the mechanisms of action of these genes in organ development, function, and disease processes.

Disease Model

By knocking out genes associated with specific diseases, organoid models can exhibit phenotypic features related to the disease, helping researchers gain a deeper understanding of the disease's mechanisms and discover new therapeutic targets.

Drug Discovery Model

Knock-out organoid models that simulate disease states are closer to reality and can be used to test the targeted efficacy of drugs. This helps accelerate the drug development process and reduces reliance on animal models.

Precision Therapy Research Model

Knock-out organoid models based on patient-specific gene knockouts contribute to studying functional changes in specific genes of individual patients. This provides valuable information for the selection and optimization of precision therapy approaches.

Literature Sharing Based on Knock-out Organoid Models

• Title: Generation and multiomic profiling of a TP53/CDKN2A double-knockout gastroesophageal junction organoid model
• Background: TP53 and CDKN2A are key tumor-suppressor genes, and their loss of function may occur early in gastroesophageal junction adenocarcinoma (GJA). However, due to the lack of disease models specifically designed for studying GJA, the understanding of the pro-oncogenic effects of TP53 and CDKN2A in GJA remains limited.
• Model Used: TP53/CDKN2A double-knockout gastroesophageal junction organoid model
• Research Findings: The concurrent deficiency of TP53 and CDKN2A leads to cellular transformation in the gastroesophageal junction, promoting accelerated growth. Moreover, implantation of this knockout organoid model results in tumor formation in immunocompromised mice. Subsequent lipidomic analysis reveals that platelet-activating factor (PTAF), associated with TP53 and CDKN2A inactivation, may play a crucial role in carcinogenesis. Inhibition of PTAF significantly suppresses the formation, proliferation, and further progression of cancer cells in this gene-knockout organoid model.

Fig.1 Knockout of TP53/CDKN2A promotes neoplastic transformation in human normal gastroesophageal junction organoids.1

Knock-out Organoid Model Construction and Functional Research Services at Creative Biolabs

Service Advantages:

• Rich Construction Experience
• Customized Solutions
• Advanced Technology Platforms
• Multidisciplinary Applications

Service Process:

• Acquisition of organoid sources
• Gene knockout
• Organoid generation
• Identification and screening
• Phenotypic studies
• Target regulatory network research

Creative Biolabs provides comprehensive gene knock-out organoid model construction and functional research services. Our professional team and advanced technologies ensure high-quality experimental results. We provide excellent support to our clients through customized solutions, accurate data analysis, and reports. If you are seeking a reliable partner to advance your research and drug development, please contact us immediately for outstanding services. In addition to gene knock-out, we also offer over-expression organoid models, knock-down organoid models, and knock-in organoid models to meet your comprehensive target research needs.

Related Sections:

• Gene Editing Organoid Based Target Analysis and Validation Services
• Off-Target Screening Cell Microarray Assay