Patient-Derived Colorectal Cancer Organoid Model

Colorectal cancer (CRC) is a malignant tumor of the digestive system. The incidence of colorectal cancer is increasing yearly, severely affecting human digestive tract health around the world. Creative Biolabs keeps an eye on this disease and continuously develops optimumin vitro models like patient-derived colorectal cancer organoid models to support your CRC research.

Construction of Patient-derived Colorectal Cancer Models

Patient-derived CRC models can be established from the tumor tissue of the colon. As shown in Fig.1, tumor tissues from the CRC patient are digested to obtain the cells and crypts, which are seeded with Matrigel in a culture plate. Then, the organoid culture medium is added to help form the 3D fine structure of the organoids. After homology identifications, such as HE staining, and whole genome immunohistochemistry, the patient-derived organoid (PDO) model can be used to test clinical therapy and construct living biobanks.

Fig.1 Development of patient-derived colon cancer organoid. (Parseh, 2022)

Limitations of Common Colorectal Cancer Models

It's been a long time since cell lines and animals were used as research models for colorectal cancer. Many researchers have found that these common models have obvious limitations, especially the inability to reflect the cancer progression in vivo. Cell lines can’t retain the tumors' microenvironment and are powerless to preserve genetic information and heterogeneity after being passaged multiple times. Animal models are superior to 2D cell cultures in many ways, but the tissue microenvironment is still different from humans. Furthermore, they are not very satisfying in terms of cost, modeling cycle, and success rate. So, it is necessary to construct a novel model for CRC research.

Advantages of Patient-derived Colorectal Cancer Organoid Models

The emergence of patient-derived organoids (PDOs) brings a new horizon for bridging the gap between animal models and humans. This recently-developed colorectal cancer PDO model displays a variety of advantages over 2D cell lines and animal models.

• Firstly, this model is able to retain 3D architecture and complexity similar to in vivo microenvironment.
• Secondly, these organoid models have a high success rate.
• Thirdly, such models are very appropriate for constructing a biobank prepared to conduct a series of research.
• Last but not least, as a good platform for studying tumorigenesis, such models could be informative for indicating the gaining of malignant potential.

Related Services

As the technology continues to mature and develop, these CRC PDO models have various popular applications, especially for preclinical drug screening and prediction of patient response to specified treatment options. Creative Biolabs is a global biological company committed to providing 3D PDO tumor models in response to various demands of the life sciences and pharmaceuticals. With rich research experience and a prominent expert network, we look forward to offering reliable patient-derived colorectal cancer organoid models and related technology services along the science research path. Don’t hesitate to contact us for professional technical support.

Related Sections:

• Patient-Derived Organoid (PDO) Model
• PDX-Derived Organoid (PDXO) Tumor Model