PDX-Derived Breast Cancer Organoid Model

Breast cancer (BC) is a serious threat to female health, and there is no specific medicine for its treatment currently. Creative Biolabs has developed PDX-derived breast cancer organoid models for the exploration of potential therapeutic strategies against breast cancer.

Shortcomings of Common Models

Commonly used cell models are passaged in a 2D medium and fail to normally describe the structure and microenvironment of tissues in vivo. In addition, the cell lines often undergo genetic drift and lose the heterogeneity of the original tumor. By contrast, as a supplement to the in vitro 2D model, PDX models perform well in most cases. But the expensive cost and long period are hard to accept. Besides, its adaptability to the high-through screening of such models is not quite satisfying.

Advantages of PDX-derived Breast Cancer Organoid Models

Recently developed organoid models offer new horizons to model and analyze patient samples, and are increasingly becoming a novel tool to further characterize the molecular features of BC. Compared to traditional cell and animal models, their advantages in many aspects are overwhelming.

• Such models are relatively easy to initiate, maintain and expand ex vivo, as well as, easy to manipulate genetically.
• This type of culture system makes it possible to monitor cell-cell and cell-matrix interactions.
• They are an ideal tool for high-through drug screening due to the moderate cost, and can be also used to study the upsetting drug resistance issue.
• The features involved in histology, genomics and transcriptomics are captured in PDX-derived organoids.
• It makes it possible to reproduce the original tumor heterogeneity in vitro.
• It’s realizable to co-culture organoids with multiple types of cells to analyze associations between infectious agents, the immune system and cancer progression.

Applications

Breast cancer organoids including PDX-derived BC organoids have been applied in large-scale drug screening, evaluating the drug sensitivity and validating disease-causing genomic variations as a complementary model. This technology will exist for a long time and boost the scientific advances in revealing the evolution mechanism of tumors and discovering new therapeutic drugs.

Fig.1 Main applications of breast cancer organoids. (Creative Biolabs)

Related Services

As a global biology company, Creative Biolabs is sensitive to advanced technology and committed to providing 3D organoid models and reliable services listed below to customers worldwide in the breast cancer field. At Creative Biolabs, we pursue the most advanced science and take every customer and your project seriously. Whenever you need it, we will provide the highest quality PDX-derived breast cancer organoid model to facilitate your preclinical drug discovery projects. Please contact us at any time for any professional technical support you want.

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

Related Sections:

• Patient-Derived Organoid (PDO) Model
• PDX-Derived Organoid (PDXO) Tumor Model