PDX-Derived Cholangiocarcinoma Organoid Model

Cholangiocarcinoma (CCA) is a common hepatic malignancy characterized by a poor prognosis. Due to the lack of drugs without resistance, the current treatment has limited effectiveness. Creative Biolabs attempts to explore several novel research tools for this disease field and has successfully built PDX-derived cholangiocarcinoma organoid models to help the research on CCA.

Shortcomings of Common Models

In conventional cell models, the system is short of the tumor microenvironment (TME) and can’t represent the complexity of in vivo environment. Long-term passage will lead to genetic variance. For patient-derived xenograft (PDX) models, sample accessibility, economic issues, and ethical concerns hinder their further development and applications. So, it’s urgent and necessary to create more representative preclinical models to boost new drug research and development.

Advantages of PDX-derived Cholangiocarcinoma Organoid Models

Organoids are micro-organs growing in vitro culture conditions and keep high similarity to tumor tissue in vivo in structure and functions. CCA tumoroids derived from the PDX mouse model are established by the disassociated tumor tissues. They possess excellent performances in other aspects except for the combination of the features of common models. The specific advantages can be concluded as follows.

• The organoids recapitulate the heterogeneity of original tumors, showing promising potential in studying drug resistance.
• This ex vivo organoid model maintains the genetic and histopathological characteristics of the original tumor.
• They relatively precisely display semble in vivo responses to chemotherapeutic compounds in vitro,which offers a bridge between clinical and non-clinical research.
• Different from PDX models, the CCA organoid construction with a high success rate requires less time and less tissue, and can stably show the key characteristics of primary tumors even after long-term passaging.
• Furthermore, gene modification is easy to operate in such models than in vivo, such as genetic knockouts via gene editing technology.

Applications

Until now, CRC organoids have become an ideal platform for both basic research and translational medicine, and are gradually applied in many life science research.

Fig.1 Main applications of PDX-derived cholangiocarcinoma organoids. (Creative Biolabs)

Related Services

As a global biology company, Creative Biolabs keeps track of the most advanced technology and is committed to developing 3D organoid models responding to the CRC research needs of customers worldwide. At Creative Biolabs, we take every customer and your project seriously and expect to become your first choice. Whenever you need it, we will offer the highest quality PDX-derived cholangiocarcinoma organoid model and related services listed below to facilitate your preclinical drug discovery process. Please contact us at any time for any professional technical support you want.

Related Sections:

• Patient-Derived Organoid (PDO) Model
• PDX-Derived Organoid (PDXO) Tumor Model