PDX-Derived Gallbladder Cancer Organoid Model

Gallbladder cancer (GBC) is one of the most lethal diseases worldwide. The concealment feature of GBC in the atypical stage results in that surgical resection is almost impossible at the time of diagnosis in most cases. To promote the life quality of patients, it is necessary to further strengthen basic research and clinical research to improve the prognosis. At Creative Biolabs, we have built a professional team to study new 3D modeling technologies such as PDX-derived GBC organoids to serve our customers.

Shortcomings of Common Models

Prior to organoids, three main models used for GBC research include 2D or 3D cell culture and xenograft models. 2D cell culture system has revealed tumor invasive growth. Nevertheless, they are easy to accumulate mutations and fail to recapitulate the in vivo interactions between tumors and surrounding cells. 3D cell cultures lose the histological and morphological characteristics of tumors. Patient-derived xenograft (PDX) models partially reproduced in vivo pathological features. However, they show a different tumor microenvironment (TME) from the parental tumor. Besides, the long establishment cycle, low success rate and ethical controversy limit more extensive applications.

Advantages of PDX-derived Gallbladder Cancer Organoid Models

The organoid models are also one of the 3D cell cultures. Recently developed novel PDX-derived GBC organoid models can grow into mini self-organizing tissues in vitro, showing certain advantages over common GBC models.

• The 3D cell culture system simulates TME and retains key features including histological features, expression profiles, and unique markers.
• They can be stably cultured and passaged for a long period in a 3D environment.
• They can retain high heterogeneity of the parental tumor in many aspects, which is appropriate for high-throughput drug screening.
• To overcome the lack of an intact immune system, such models can combine CRISPR/Cas9 gene editing to establish a more applicable model under some circumstances.

Fig.1 Timeline of the development of different models of gallbladder cancer cell culture. (Huang, 2021)

Applications

At present, it has already become possible to apply PDX-derived GBC organoid models in the mechanism research involving the occurrence and development of tumors, drug screening, drug sensitivity testing, tumor biology as well as the establishment of a personalized GBC immunotherapy model.

Related Services

Over the later decades, Creative Biolabs is focused on the 3D biology technology area and can capture frontier information. To date, we have built the ex vivo physiological model network containing rich 3D organoid products, which are highly customizable and provide a beneficial tool for our customers to study various tumor behaviors. With rich experience and professional scientific team, we’re ready to offer high-quality PDX-derived GBC organoid model and related preclinical drug discovery services listed below to ensure a successful advancement of your drug discovery project. If you’re interested in more detailed information, please feel free to contact us.

Related Sections:

• Patient-Derived Organoid (PDO) Model
• PDX-Derived Organoid (PDXO) Tumor Model