Creative Biolabs is a world-leading service provider of application-specific antibody development. Here, we introduce our in vitro diagnostic (IVD) antibody development and immunoassay development services targeting E-Selectin marker. We are confident that our commitment to science and research will enable us to offer you the best products and services.
E-Selectin
E-selectin is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. It is a protein that in humans is encoded by the SELE gene. Like other selectins, it plays an important role in inflammation. In the course of inflammation, E-selectin is essential in recruiting leukocytes into the site of injury. Macrophages locally release cytokines IL-1 and TNF-α in inflamed tissues to induce E-selectin overexpression on endothelial cells adjacent to blood vessels. Leukocytes in the blood expressing the correct ligand will bind E-selectin with low affinity, also under shear stress of blood flow, causing the leukocytes to "roll" along the inner surface of the blood vessel as the temporary interaction are being destroyed.
Fig. 1 Structure of the SELE protein.1
E-Selectin Marker of Sepsis Infections
Soluble isoforms of E-selectin can be found in the supernatant of endothelial cells cultured in vitro within 24 hours of cytokine activation and are generated through a largely caspase-dependent shedding process. In healthy individuals, low levels of soluble E-selectin (sE-selectin) are found in serum, but these levels are greatly elevated in septic patients. Importantly, shed sE-selectin from sera of septic patients retained the ability to adhere to granulocytes in vitro. Shedding of E-selectin has thus been proposed to limit leukocyte-endothelial interactions both by decreasing the cell surface density on the endothelium and by generating an intravascular competitive inhibitor or decoy ligand (that is, sE-selectin) for leukocytes, thereby reducing collateral damage in the host. Indeed, one clinical study found that while sE-selectin was elevated in septic children, those with the highest levels exhibited the best outcomes and survival rates.
Five soluble adhesion molecules are associated with sepsis, including E-selectin, L-selectin and P-selectin, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1. While increased levels of these soluble adhesion molecules generally correlate well with the presence of sepsis, their degree of elevation is still poorly predictive of sepsis severity scores, outcome and mortality. Separate analyses of neonates, children, and adults demonstrate significant age-dependent discrepancies in both basal and septic levels of circulating soluble adhesion molecules.
IVD Antibody Development Services Targeting E-Selectin Marker
In recent years, IVD technology is undergoing rapid development. IVD antibodies have been widely used in immunodiagnostic tools to aid in the diagnosis of various diseases, conditions, and infections. With years of experience and advanced technology, Creative Biolabs is able to provide a full range of high-quality and cost-effective biomarker-specific antibody development services. Especially, we help develop novel anti-E-Selectin antibodies for your IVD projects. Having completed a number of IVD antibody generation and development projects, Creative Biolabs is confident to offer you the best products and services to promote your project a success.
If you are interested in the services we can provide, please don't hesitate to contact us or directly send us an inquiry.
Reference
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