Creative Biolabs is one of the leading custom antibody generation and development providers. We specialize in the generation of antibodies against a wide range of targets for R&D, diagnostic, and therapeutic applications. Currently, we have launched a series of in vitro diagnostic (IVD) antibody development services targeting numerous diagnostic biomarkers of human diseases. Here, we focus on the F2 Isoprostanes.
General Introduction of F2 Isoprostanes
The oxidation of cellular lipids typically referred to as lipid peroxidation, is a central feature of oxidant stress, a phenomenon that has been increasingly implicated as playing a causative role in the pathophysiology of a number of human diseases. Lipid peroxidation, which is typically initiated by highly reactive free radical species, can be assessed by many methods including the measurement of either primary or secondary peroxidation end products. Primary end products of lipid peroxidation include conjugated dienes and lipid hydroperoxides, while secondary end products include thiobarbituric reactive substances (TBARS), gaseous alkanes and a group of prostaglandin (PG) F2-like products termed F2-isoprostanes (F2-IsoPs). Quantification of these various compounds has proven highly useful for the study of free radical-mediated lipid peroxidation in the in vitro model systems. Recall that oxidative stress is a key process in the development of CAD, diabetes, hypertension, and obesity.
Fig. 1 The structure of F2-Isoprostanes (8-iso-PGF2a).
The Role of F2 Isoprostanes in Sepsis
As reported, F2-isoprostanes are markers of oxidant injury in vivo. Numerous studies have indicated that these compounds are extremely accurate measures of lipid peroxidation and have illuminated the role of oxidant injury in a number of human diseases including atherosclerosis, Alzheimer's disease, and pulmonary disorders. Additionally, plasma levels of F2-isoprostanes and isofurans are associated with renal, hepatic, and coagulation failure, but not with circulatory or pulmonary failure in severe sepsis. The result suggests that lipid peroxidation is a prominent feature of septic multisystem organ failure. The report has also shown that plasma isoprostanes and isofurans may play a role in monitoring oxidative stress in treatment trials of antioxidant therapies in severe sepsis.
IVD Antibody Development Services Targeting F2 Isoprostanes
Antibodies are core elements for antibody-based immunoassays like immunonephelometry and sandwich ELISA for detecting and quantifying antigens of interest in biological samples such as the serum, urine, tissue preparations. At Creative Biolabs, we offer a full range of antibody development, antigen & antibody conjugation, and IVD kit development services for our worldwide clients. We are confident to provide top-quality service to facilitate diagnostic antibody development.
A team of experienced scientists focused on antibody development are pleased to assist you in your project in a timely and cost-effective manner. For more detailed information, please feel free to contact us or directly send us an inquiry.
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