Creative Biolabs is a leading service provider that focuses on polyclonal, monoclonal, and recombinant antibody development for research, diagnosis, and potential therapeutics. Based on our extensive experience and state-of-the-art platforms, Creative Biolabs now offers a series of biomarker-specific IVD (in vitro diagnostic) antibody development services to clients globally. Especially, we introduce our IVD antibody development services for the elastase marker.
General Description of Elastase
Elastase (HLE) is a serine protease digest and degrades a number of proteins including elastin which is an elastic substance in the lungs and some other organs that supports their structural framework. HLE is produced in the pancreas as an inactive zymogen and activated in the duodenum by trypsin. The following information applies to porcine elastase. Elastases are defined by their ability to release soluble peptides from insoluble elastin fibers by a proteolytic process. Elastase is another protease that induces disintegration of the axoneme by microtubule sliding in the presence of ATP. Elastase and trypsin differ in their effects on the flagellar waveform of reactivated sperm, suggesting that they disrupt different structural components. It has been shown that elastase disrupts tight junctions, cause proteolytic damage to tissue and break down cytokines.
Fig. 1 The structure of Neutrophil Elastase.1
Elastase and Sepsis Disease
Sepsis is a systemic inflammatory response to known or suspected infection and associated with microvascular dysfunction and capillary leak. The cause of capillary leak may be due to substances released by activated polymorphonuclear neutrophils (PMNs) that adhere to microvascular endothelial cells. Following binding, PMN often degranulates releasing azurophilic enzymes like HLE and proteinase 3 (PR3)) at the endothelial surface and into plasma. These enzymes, particularly HLE and PR3, contribute to the loss of endothelial integrity. Through a mechanism involves the actions of HLE and PR3, neutrophil degranulation such as increased plasma concentrations can result in increased endothelial permeability. Neutrophil elastase plays an important role in the sepsis-induced myocardial dysfunction.
IVD Antibody Development Services Targeting Elastase Marker
IVD antibodies have been widely used for disease screening and therapeutic monitoring. As a research partner with years of experience in high-quality antibody development and production, Creative Biolabs offers one-stop solutions from antigen design to antibody pair screening. Besides, we also offer diagnostic immunoassay development services, including feasibility analysis, assay design, assay protocol establishment, assay optimization, and kit production.
Our services can be tailor-designed to adapt to specific project specifications. If you are interested in our services, please do not hesitate to contact us for more details.
Reference
For Research Use Only.
