Antibodies-based in vitro diagnostic (IVD) tools have proven to be useful in helping clinicians to diagnose or assess the progression of a variety of diseases. With years of experience in antibody development, Creative Biolabs has launched a series of IVD antibody development services targeting a wide range of disease biomarkers. The services are customized to suit the specific needs of our clients. Here, we focus on the endothelin-1 marker of sepsis.
Endothelin-1 (ET-1)
Endothelin 1 (ET-1) is a potent vasoconstrictor that in humans is encoded by the EDN1 gene. It is produced by vascular endothelial cells. The protein is proteolytically processed to release a secretory peptide called endothelin-1. Endothelin 1 is one of the three isoforms of human endothelin. Preproendothelin is a precursor of peptide ET-1. Endothelial cells convert pre endothelin to pro-endothelin, which is then converted to mature endothelin released by the cell. Endothelin-1 receptor antagonist is used to treat pulmonary hypertension. Inhibition of these receptors prevents pulmonary vasculature constriction and thus reduces pulmonary vascular resistance.
Fig. 1 The classical pathway of Endothelin-1.1,2
Endothelin-1 Marker of Sepsis Infections
ET-1 acts as a potent vasoconstrictor and has a mitogenic effect on smooth muscle cells. ET-1 has been shown to be involved in a variety of physiological functions related to the cardiovascular, gastrointestinal, renal, nervous, respiratory and endocrine systems. Due to its short half-life, about 1-7 minutes, and almost complete clearance from the bloodstream by pulmonary passage, CT-proET-1 has been found to stoichiometrically measure ET-1.
ET-1 is associated with myocardial injury and death in patients with septic shock. The concentration of CT-proET-1 is increased in non-survivors of septic shock. CT-proET-1 seems to be very important for long-term mortality. ProADM and proET-1 are particularly attractive biomarkers for a septic shock because they are both endothelium-derived pro-hormones, and their end products have important vasoregulatory opposing effects. It is plausible that the net balance between hormones is of significance for clinical outcomes. Increased concentrations of ADM and ET-1 in patients with systemic inflammatory response syndrome (SIRS) and septic shock have been described and appear to be related to severity and mortality.
IVD Antibody Development Services Targeting Endothelin-1
IVD antibodies have been commonly used in immunodiagnostic kits for the disease diagnosis and prognosis. Creative Biolabs has built a versatile IVD platform to provide custom biomarker-specific IVD antibody development services to global clients. Along with over a decade of rich experience in providing excellent custom services for IVD antibody discovery, our services can totally meet your special requirements.
In addition, Creative Biolabs provides one-stop diagnostic immunoassay development services, including feasibility analysis, assay design, assay protocol establishment, validation, and production. If you are interested in our service, please feel free to contact us for more information and a detailed quote.
References
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