Cardiotoxicity Evaluation by iPSC-derived Cardiomyocytes

The primary challenges in contemporary cardiac research stem from the scarcity of tissue-specific cells available for in vitro modeling and the direct acquisition of cardiomyocytes from patients. By utilizing cardiomyocyte clusters derived from human induced pluripotent stem cells (iPSCs), Creative Biolabs provides a mature alternative for cardiotoxicity evaluation and drug research, offering a novel and reliable strategy for chemical safety assessment.

iPSC-derived cardiomyocytes. (Rojas, 2017) Fig 1. iPSC-derived cardiomyocytes.¹

Cardiotoxicity Analysis Based on iPSC-derived Cardiomyocytes Creative Biolabs' preclinical cardiac safety testing is a cell-based detection method that provides more effective predictive information than traditional cardiac toxicity tests through optimized and well-characterized assay procedures. Any modifications to the testing process are feasible and can be discussed with our technical staff.
Detection Tools	Cell Types	Testing Protocol	Data Output
We employ a microelectrode array (MEA) to effectively measure concentration-dependent electrophysiological responses of spontaneously active cells and evaluate changes in major stereotypical channels associated with action potentials.	We utilize iPSC-derived cardiomyocytes for plating, which exhibit spontaneous beating, express cardiac-specific markers, and demonstrate strong consistency with in vivo or ex vivo cardiac effects of various compounds.	The default testing protocol involves incubation with five sequentially graded concentrations, with two replicates for each condition. Electrophysiological information at a single time point will be obtained for reproducible data.	In addition to detailed experimental procedures, you will receive comprehensive electrophysiological data and compound risk assessment reports, including potential amplitude, conduction velocity, field potential duration, pulse frequency, and beat count.

Choosing Creative Biolabs, You Will Benefit From:

Comprehensive morpho-functional analysis
MEA detection provides comprehensive, complete, and highly accurate electrophysiological reports, while contractility testing (impedance measurement) or molecular biology detection is also within our capabilities.
High reproducibility
iPSC-induced cardiomyocytes possess characteristic features of human cardiac cells, stably expressing myocardial troponin-I after forming electrically connected syncytial layers, and exhibit expected electrophysiological/biochemical responses to drug exposure.
Optimized gene purification schemes
Optimized differentiation schemes maximize the yield and purity of cardiomyocytes, allowing specific experimental targets to carry specific mutations.

Our Services

Creative Biolabs is committed to providing unique and comprehensive in vitro cardiotoxicity evaluation services for preclinical drug discovery. Depending on your screening targets and experimental needs, we have deployed cardiac toxicity prediction services and safety assessment strategies based on different models.

Cardiotoxicity Evaluation by Cardiac Organoid Model

Cardiotoxicity Evaluation by Precision-Cut Human Heart Slice Model

Cardiotoxicity Evaluation by 3D Cardiac Spheroid Model

Cardiotoxicity Evaluation by Ex vivo Human Cardiovascular Tissue Model

Cardiotoxicity Evaluation by iPSC-derived Cardiomyocytes

hERG Safety Evaluation

Creative Biolabs would like to extend a personal invitation for you to contact us. At Creative Biolabs, we pride ourselves on fostering meaningful collaborations that drive scientific progress.

References

Rojas, S.; et al. Transplantation of purified iPSC-derived cardiomyocytes in myocardial infarction. PLOS One. 2017, 12(5): e0173222.
Nijak, A.; et al. Morpho-functional comparison of differentiation protocols to create iPSC-derived cardiomyocytes. Biology Open. 2022, 11: 59016.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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