3D Ex Vivo Human Acetylcholine/Carbachol Model Introduction

Diseased 3D ex vivo secondary airways need to receive certain stimuli to produce a contraction response. Therefore, suitable stimuli are crucial for the validation of drug candidates in the 3D ex vivo secondary airways (diseased) contractility model. Creative Biolabs provide acetylcholine or carbachol-induced 3D ex vivo secondary airways (diseased) contractility model to help customers characterize the effect of drug candidates.

Acetylcholine/Carbachol-Induced Second Airways Constriction Model

Both acetylcholine and carbachol are agonists of the muscarinic acetylcholine receptor (mAChR), and they are neurotransmitters that play important roles in the central and peripheral nervous systems. When acetylcholine or carbachol binds to mAChRs located on airway smooth muscle cells, it triggers a phosphorylation cascade signaling pathway that ultimately leads to smooth muscle cell contraction and subsequent airway constriction. However, acetylcholine is rapidly degraded by the enzyme acetylcholinesterase present in lung tissue, so its contractility effect is short-lived. Carbachol is a synthetic cholinergic agonist that is structurally similar to acetylcholine but more resistant to enzymatic degradation. Therefore, carbachol is more potent and has a longer duration of action than acetylcholine in inducing constriction of the airways in the lungs.

Fig.1 Schematic illustration of CCh-induced endogenous TGF-β1 signaling loop in COPD lung fibroblasts. (Namba, 2017)

In summary, the 3D ex vivo secondary airways (diseased) contractility model induced by acetylcholine and carbachol can be used to study the contraction and relaxation of airway smooth muscle in diseased lungs. This model has several advantages, including:

• More actual
  Compared with in vivo animal models and in vitro cell models, this model is closer to the actual pathological conditions of the human body.
• More rigorous
  To provide a reference for determining whether your drug candidate causes relaxation of the second airway through acetylcholine receptors.
• More reliable
  High reproducibility due to the stable effect of airway constriction induced by acetylcholine or carbachol.

Creative Biolabs has been expanding the range of 3D ex vivo disease models for drug discovery and development. If you have been searching for the most suitable acetylcholine or carbachol-Induced 3D ex vivo secondary airways (diseased) contractility model, please feel free to contact us. Our professional technical team will provide you rigorous verification scheme in the most cost-effective way. For the 3D ex vivo secondary airways (diseased) contractility model, we also provide following models.

• 3D Ex Vivo Human Adrenoceptors-Isoprenaline Model
• 3D Ex Vivo Human COPD Model

Related Sections:

• Ex Vivo Secondary Airways (Healthy) Contractility Model
• Ex Vivo Secondary Airways (Diseased) Contractility Model
• Ex Vivo Tertiary or Lower Airways (Healthy) Model
• Ex Vivo Tertiary or Lower Airways (Diseased) Model
• Ex Vivo Tertiary or Lower Airways (Bronchodilation) Model