Alcoholic Fatty Liver Disease-on-a-Chip Model Introduction

Creative Biolabs provides alcoholic fatty liver disease-on-a-chip model with cutting-edge technology to control the microenvironments of cells, including critical microarchitecture, spatiotemporal cell-cell interactions, and extracellular physiological flow.

Formation of Alcoholic Fatty Liver Disease-on-a-chip Model

The alcoholic fatty liver disease chip model contains two polymethyl methacrylate substrates, three PDMS layers, and two pieces of polycarbonate membranes to form three chambers. The two sides of the top and bottom polycarbonate membranes are seeded with hybridoma cells and human hepatic stellate cells respectively to simulate the endothelial barriers. After 2h for the cells to attach to the PC membranes, a multi-channel peristaltic pump was connected at the inlet of a chip to provide nutrition and shear conditions for each type of cell cultured in the chip.

Fig 1. Schematic of liver sinusoid structure and the liver-on-a-chip microdevice. (Deng, 2019)

To complete perfusion culture and control the interstitial-level flow rate of media, we use a microfluidic chip by exposing spheroids to the medium containing different concentrations of ethanol for 48 hours. Spheroids treated with ethanol are cultured with fresh medium to mimic the recovery process of the injured liver.

Fig 2. Schematic depiction of in vitro ALD model. (Lee, 2016)

Advanced Alcoholic Fatty Liver Disease-on-a-chip Model at Creative Biolabs

• Involvement of multiple non-parenchymal cells
• Perfusion culture conditions
• Mimicking the complex structure and microenvironment of hepatic in vivo

Applications of Alcoholic Fatty Liver Disease-on-a-chip Model

• Recreate the damage process of hepatic non-parenchymal cell lines induced by alcohol.
• Measure multiple biomarkers of each type of hepatic non-parenchymal cell lines.
• Serve as a functional platform for drug-induced hepatotoxicity, liver function, and disease.
• Potential for further studies such as pathological analysis of ALD and drug- and toxicity-screening.

Why Choose Us

Our alcoholic fatty liver disease-on-a-chip model allows facile observation of structural and functional changes in microtissues in situ. It has the potential to be used as a drug-screening tool and to facilitate the study of hepatotoxicity and liver disease-related mechanisms.

Apart from the alcoholic fatty liver disease-on-a-chip model, there are other models that might capture your interest:

Related Services

Elevating our collection of disease-on-a-chip models, Creative Biolabs launched a suite of specialized technical solutions, aiming at offering one-stop services to meet the diverse research needs of our global clients. Reach out to us to explore the capabilities of our highly customizable models and get a tailored quotation.

Related Sections:

• Single Organ-on-a-Chip Model Introduction
• Multiple Organs-on-a-Chip Model Introduction
• Barrier Tissue On-a-Chip Model Introduction
• Customized Tissue-on-a-Chip Model Introduction
• Disease-on-a-Chip Model Introduction