Kidney-on-a-Chip Model Introduction

Kidney toxicity represents the most common adverse event resulting from drugs, and its assessment is critical for drug development. Creative Biolabs offers Kidney-on-a-chip Model to help researchers in examining the work of the proximal tubule and see how it works when damaged. Our kidney-on-a-chip model will push the study of chronic kidney diseases, which are steadily increasing with the increased prevalence of diabetes, hypertension, and old age.

Glance of Kidney-on-a-chip

Multiple cell types and functional units comprise the human kidney. Thus, to be physiologically relevant, our biomimetic kidney-on-a-chip has integrated cell-cell interactions, such as those that exist between glomerular vascular endothelial cells and podocytes, transcellular electrochemical and osmotic pressure gradients, the structural arrangement of renal tubular segments, fluid flow dynamics, and cellular metabolic and endocrine functions.

Fig 1. Future of drug testing. (Ashammakhi, 2018)

Creative Biolabs' Microfluidic Kidney-on-a-chip Platform

The conventional culture system mimics crossepithelial transport but lacks fluid flow and the apical function of in vivo–like kidney functions. Creative Biolabs' kidney-on-a-chip is a fluidic-flowing device consisting of a structural component made of polydimethylsiloxane, and a microfluidic component either attached to a pump or syringe with constant flow pressure. The apical surface of kidney proximal tubular epithelial cells (PTECs) is exposed to constant glomerular flow. Compared with other in vitro models, our kidney-on-a-chip platform displays the following unique advantages.

• Evaluating drug efficacy with the representation of in vivo environment, wherein different structures and cells interact with each other, and the difference in drug pharmacokinetics and pharmacodynamics response between animals and humans.
• Showing great promise in recapitulating native tissues in vitro to study pathology and characterize potential drugs.
• Mimicking the structural, mechanical, transport, absorptive, and physiological properties of the human kidney and reproducing a 3D microenvironment to create a suitable in vitro system for drug-induced kidney injury.
• Advanced developments of the structural-functional arrangements of different nephron segments, the specialized structures resulting from these arrangements, the physiology of countercurrent exchange, and the diffusion of nutrients, waste, hormones, and drugs through the interstitium of the native kidney.

Fig 2. Fabrication and operation of a proximal tubule on a chip. (Jang, 2010)

Applications

Kidney-on-a-chip has various potential applications. Fabricating disease model in the kidney chip can be simply done when the identification of diseases mechanism and antigens are offended. Using pharmacokinetic data from animal models bears the uncertainty of underrating human nephrotoxicity. The usage of our kidney-on-a-chip may fill the gaps through management of drug absorptions and flow rates that duplicate human drug evacuation or metabolism. Additionally, kidney-on-a-chip has also been considered as an important compartment of an eventual multiorgans-on-a-chip system.

Our Capabilities

Creative Biolabs' capability in mimicking the interaction between blood vessels and tubular cells, as occurs in the juxtaglomerular apparatus and in countercurrent exchange, lays an important foundation for Kidney-on-a-chip Model design and construction. Our microfluidic systems mimic the in vivo microenvironment including multi-compartmentalization and exposure to fluid shear stress. The improved 3D microfluidic systems are placed in the perspective of investigating drug-induced kidney toxicity.

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Related Services

As a prominent global biology enterprise, Creative Biolabs keeps pace with cutting-edge technology and is firmly committed to providing dependable services listed below to clients worldwide, aiming to impel preclinical drug discovery projects towards resounding success. If you have any special needs in organ-on-a-chip model or be interested in learning more about Creative Biolabs' 3D biology-based services, please feel free to contact us for more details.

Related Sections:

• Single Organ-on-a-Chip Model Introduction
• Multiple Organs-on-a-Chip Model Introduction
• Barrier Tissue On-a-Chip Model Introduction
• Customized Tissue-on-a-Chip Model Introduction
• Disease-on-a-Chip Model Introduction