Normal Cortical Organoid Model

Cortical organoids are increasingly expanding their application range owing to the capability of simulating human brain structure. In recent years, Creative Biolabs also pay attention to 3D ex vivo models and has established various tissue-derived normal organoids. Normal cortical organoids (NCOs) are one of our flagship products, which will pave the way for your preclinical therapeutic strategy development project.

Construction of Normal Cortical Organoid Models

At present, Cho et al. have successfully built the NCO model starting from human embryonic stem cells (hESCs) based on previous research. The protocol is composed of three main steps: culture, aggregation and differentiation, as shown in Fig.1. Via the 3D culture system, they demonstrated the feasibility of culturing human cortical organoids (hCOs) in the in vitro environment and gave confidence to extensive applications in brain disease studies.

Fig.1 Establishment of hESC-derived cortical organoid. (Cho, 2022)

Advantages of Normal Cortical Organoid Models

NCOs provide considerable advantages due to their visual nature and operability in terms of either molecular or cellular manipulations, which has aroused deep interest in the academic world.

• Firstly, they capture the cell ingredient and function of the human brain, and create a new vision of studying the progress process related to brain diseases.
• Such models highly match the research needs of multiple kinds of neurological disorders because they possess the ability to represent cell-cell interactions in a similar in vitro environment to in vivo.
• In addition, such models are easy to manipulate in response to a variety of needs, such as exploring the assembly of neural circuits and interactions between various types of cells.
• By creating a suitable growth condition, they can be cultured and maintained in vitro in a robust and reproducible manner for a long time.

Applications of Normal Cortical Organoid Models

Organoids are an ex vivo model that can be rapidly established and their easiness has been verified by many reports. With the advent of gene-editing technology, it has become more convenient and easier to accomplish genome manipulation and the genetic control of brain development in humans. By reducing the use of transgenic animals, they further simplify the modeling operation when testing how gene mutations influence brain development. According to the research result of Cho et al., hCOs offer a dynamic platform for drug screenings for complex neurological diseases, which is attributed to the presence of active neuronal networks. In light of the above, we can foresee that NCOs will play an essential role in brain disease research.

Related Services

Creative Biolabs is leading in providing 3D ex vivo physiological models. With advanced technology platform and experienced scientific team, we can provide the most advanced organoid products including NCOs and professional technical services to our global customers. If you’re interested in our services, please contact us in time to discuss your project for professional assistance.

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