3D Ex Vivo Human IBD Permeability Model Introduction

The intestinal tract has received increasing attention due to its important role in human metabolism. Dysregulation of gut function contributes to the development and spread of several gut diseases that affect millions of people worldwide, especially inflammatory bowel disease (IBD). Therefore, the research on the pathogenesis of IBD has become a hotspot. The study of IBD requires models that closely mirror the characteristics of the intestinal epithelium in vivo. In recent decades, Creative Biolabs has developed a variety of ex vivo human IBD permeability models to study different intestinal epithelial functions and metabolism.

Drawbacks of Traditional Models

IBD is a relapsing gastrointestinal disorder. For decades, scientists have struggled to understand its etiology and pathology. Therefore, many in vivo animal models and in vitro cell lines have been established to investigate its etiology and identify effective therapeutic strategies. Different models have certain limitations. Although in vitro cell models have proven valuable for uptake and permeability screening, they suffer from significant drawbacks that make them difficult to use as a one-stop permeability screening tool. Although animal models can simulate the physiological functions of whole organisms and are widely used, there are still some shortcomings.

Establishment of Ex Vivo model

These limitations require the development of ex vivo 3D human intestinal tissue models to correctly recreate the conditions. Crucial to ex vivo models are that the tissue is removed from its natural environment. The general principle is to install a mucous membrane between two half-chambers filled with continuously oxygenated buffer. A permeability probe was added to the buffer in the mucosal compartment, and after defined time intervals, samples were collected from the serosal compartment as measurements of passage. Ex vivo permeability models facilitate the understanding of some key aspects of IBD.

Fig.1 Strategies for the assessment of gut permeability. (Schoultz, 2020)

Why Choose Ex Vivo Human IBD Permeability Models?

Fig.2 Features of the models. (Creative Biolabs)

At Creative Biolabs, we utilize proprietary methods and advanced equipment to maximize the preservation of physiological function in ex vivo models. These models have been used to study gastrointestinal physiology, intestinal epithelial function, host-microbiome interactions, and drug absorption. If you are interested in our ex vivo human IBD permeability models, please contact us in time for more details.

More models are available through the following links:

• 3D Ex Vivo Human IBD Anti-inflammatory Model
• 3D Ex Vivo Human Healthy GI Permeability Model
• 3D Ex Vivo Human Healthy GI ion-channel Model
• 3D Ex Vivo Human Healthy Gastrointestinal Metabolism Model
• 3D Ex Vivo Human Colon Tissue Model

Related Sections:

• Ex Vivo Bile Duct Model
• Ex Vivo Esophagus Model
• Ex Vivo Gallbladder Model
• Ex Vivo Larynx Model
• Ex Vivo Pharynx Model
• Ex Vivo Palate Model
• Ex Vivo Liver Model
• Ex Vivo Pancreas Model
• Ex Vivo Parotid Gland Model
• Ex Vivo Salivary Gland Model
• Ex Vivo Stomach Model
• Ex Vivo Intestine Model