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Size | Qty | Add To Basket |
---|---|---|
500 mL | ||
1 L |
Product Description | McCoy's 5A media for SILAC is a specialized cell culture medium tailored for stable isotope labeling by amino acids in cell culture (SILAC) applications. This medium is formulated without certain essential amino acids, such as lysine and arginine, allowing researchers to incorporate isotopically labeled versions of these amino acids into proteins during cell growth. By cultivating cells in this medium supplemented with heavy isotope-labeled amino acids, proteins within the cells become labeled, facilitating their detection and quantification using mass spectrometry. McCoy's 5A media supports the growth of cell lines that require its unique nutritional composition, making it ideal for studies involving these cells. |
Synonyms | McCoy's 5A SILAC medium; McCoy's 5A nutrient medium for SILAC; McCoy's 5A for stable isotope labeling; McCoy's 5A cell culture medium for SILAC |
Form | Liquid |
Storage | Store at 2-8°C for long term storage. |
Application | McCoy's 5A media for SILAC is a specialized cell culture medium designed for SILAC. Its application is pivotal in quantitative proteomics, enabling the analysis of protein expression levels, post-translational modifications, and protein interactions under various experimental conditions. This approach enhances the accuracy and reproducibility of proteomic analyses, contributing valuable insights in fields such as cell biology, oncology, and biomarker discovery. |